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Related post: PROJECT NUMBER ZOl AI 00244-04 LPD PERIOD COVERED October 1, 1984 to September 30, 1985 TITLE OF PROJECT (80 characters or less. Title must fit on one line between the borders.) Developmental Adaptations of Trypanosoma cruzi to the Vertebrate Immune System PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation) PI: A. Sher Section Head LPD, NIAID Others: L. V. Kirchhoff K. Joiner D. Sacks Medical Staff Fellow Senior Investigator Senior, Staff Fellow LPD, NIAID LCI, NIAID LPD, NIAID COOPERATING UNITS (if any) Wellcome Biotechnology LTD, Verapamil Gel Kent, England (D. Verapamil 180 Snary) LAB/BRANCH Laboratory of Parasitic Diseases SECTION Immunology and Cell Biology Migraine Verapamil Section INSTITUTE AND LOCATION NIAID, NIH, Buy Verapamil Bethesda, Maryland 20205 TOTAL MAN-YEARS: 1.4 PROFESSIONAL: 1.2 OTHER: 0.2 CHECK APPROPRIATE BOX(ES) D Verapamil Er (a) Human subjects D (a1) Minors D (a2) Interviews D (b) Hunnan tissues ffl (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) In this project, we have been studying developmental Verapamil Hydrochloride adaptations of Trypanosoma cruzi to the vertebrate host, and in particular, surface membrane changes occuring during the morphogenesis of epimastigotes (vector stage) to metacyclic trypomastigotes (vertebrate stage). During the report period, we continued to investigate the mechanism by which metacyclic trypomastigotes become resistant to lysis by the alternative pathway of complement (ACP). Verapamil 120mg Studies with purified complement components revealed that in comparison to epimastigotes, metacyclic trypomastigotes bind minimal amounts of Factor B, an essential co-factor in the ACP. In contrast, to the findings obtained with other systems, binding of the regulatory component H was equivalent in activating Verapamil Sr epimastigotes and non-activating metacyclic trypomastigotes. Enzyme treatment studies suggested that the incapacity of metacyclic Verapamil Dosing trypomastigotes to activate the ACP was due to their elaboration of pronase and tunicamycin sensitive molecules. In related studies, we investigated the expression of a 72,000 Verapamil Dose MN Verapamil 240 Mg glycoprotein (GP72) (previously shown Verapamil Mg by us to be the acceptor for C3 on epimastigotes) on insect and culture derived metacyclic trypomastigotes. Using monoclonal antibodies, GP72 was detected on the surface of insect metacyclics but was not found on culture generated organisms. Since by surface labelling Verapamil 240 and immunoprecipitation we were able to detect Verapamil 80 Mg the molecule on this stage, it apparently is modified and expresses fewer epitopes accessible to antibody binding on intact parasites than its equivalent on epimastigotes. Finally, using rabbit anti-idiotypic antibodies directed against an anti-GP72 monoclonal antibody, we were able to Verapamil Migraine raise an antibody response against carbohydrate determinants on the GP72 molecule in mice, rabbits and guinea pigs. 10-25 PHS 6040 Verapamil Dosage (Rev. 1/84) GPO SI4-Sie DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00246-03 LPD BIOD COVERED ctober 1, 1984 to September 30, 1985 TITLE OF PROJECT (BQ chariicters or less. Title Verapamil 120 Mg must lit on one line Verapamil 80mg between the borders) Molecular Studies of the Verapamil Cream Genome and Surface of Schistosoma mansoni PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation) PT: A. Sher Section Head LPD, NIAID Others: E. J. Pearce D. Lanar T. Verapamil 240mg McCutchan Fogarty Fellow Guest Worker Senior Investigator LPD, NIAID LPD, NIAID LPD, NIAID XDPERATINP UNITS W any) Biomedical Research Institute, Rockville, MD B/BRANCH ^ „ . . Laboratory of Parasitic Diseases Immunology and Cell Biology "mmrM,^"§ethesda, Maryland 20205 TOTAL MAN-YEAR: l7 PROFESSIONA l5
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